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International Seed Testing Association
International Seed Testing Association
Richtiarkade 18
8304 Wallisellen, CH
+41 44 838 60 00
ista.office@ista.ch
International Seed Testing Association

Standard PT

The first comparative test system organised by ISTA with the aim to estimate the technical competence of member laboratories and prepare them for ISTA accreditation. It includes traditional tests that are most common in the seed testing laboratories such as purity, other seed determination, germination, moisture, and viability tests.

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Old OSD Photos

Rounds and Results

For each PT round, the testing instruction is first released, followed by the heterogeneity result and summary report.

  • PT22-3 Lolium perenne instruction

    Participants of test round PT 22-3 shall receive three samples of Lolium perenne for purity, determination of other seeds, germination and tetrazolium testing, as part of the ISTA Proficiency Test Programme for 2020-2022. Originally the moisture test was planned for this PT round but due to organizational reasons, it was cancelled.

    In case there is any doubt as to the integrity of the samples (e.g. broken seal, inadequate quantity of seeds) the laboratory is requested to contact the ISTA Secretariat.

    Please test samples and report the results in accordance with the current ISTA Rules.

    Accredited laboratories are required to participate in all tests they hold a valid accreditation.
    For non-accredited tests participation is voluntary.

    Purity Analysis

    Please note that the sample weight of the test round is based on the weight of 2500 seeds and it can differ from the weight prescribed in the ISTA Rules.

    Other Seed Determination

    The number and species names of other seeds found shall be reported in alphabetical order in accordance with the current ISTA List of Stabilized Plant Names

    Orange Certificate (OIC)

    A blank Orange International Seed Lot Certificate has been enclosed in order to give all participants the opportunity to practice reporting on OIC. It does not involve any form of rating but will help participants and ISTA to identify weak points in reporting.

    The certificate shall be filled in with results for Lot. No. 1 in addition to the reporting forms.

    When completing the Certificate, please use the following information:

    • Applicant: “ISTA Secretariat”, postal address
    • Name of the species: “Lolium perenne
    • Weight of the lot: 10,000kg
    • Category: “Certified seeds”
    • Testing and issuing laboratory: report according to the ISTA Rules
    • Sampling by: assume your lab samples and report accordingly
    • The number of containers: 250
    • Date of sampling: November 15, 2022
    • Mark of the lot: ISTA 2000 PAR

    Please report the results of all tests you performed. The certificate shall be a final certificate.

    Remaining seed shall be carefully stored for re-testing or follow-up corrective actions if required. There should be sufficient seed for one re-test.

    You are kindly asked to initiate the tests within 3 weeks after receipt, and report the results by e-mail (to pt@ista.ch).

    The deadline for reporting results is 1 March, 2023.

  • PT22-2 Spinacia oleracea heterogeneity report

    Test round 22-2 S.ole started with the shipment of three samples of Spinacia oleracea for purity, other seed identification and germination testing. Participants will receive their provisional results by e-mail.

    Samples were dispatched in July 2022 and the deadline for reporting test results to the Secretariat was 1 November 2022. By this date 92% of the 195 participants submitted their test results.

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Leader on issues encountered or specific questions arising.

    NOTE: 2 tubers were added to the Lot 2 sample and identified by many participants as other seed of Cyperus esculentus. According to the current ISTA Rules 3.3, tubers shall be classified as inert matter. This was confirmed by the ISTA Purity Committee and the identification of the Cyperus esculentus is therefore excluded from the OSD test. Reporting of 2 tubers of Cyperus esculentus was expected in the description of inert matter.

    These tubers are in some countries noxious. Therefore the participants may include them in their collection of noxious species. The images of the tubers are provided under the below link.

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr


    Heterogeneity results 22-2 S.ole
    OSD photos

  • PT22-1 Glycine max heterogeneity and summary report

    Round 22-1 G.max started with the shipment of three samples of Glycine max for germination testing. Participants will receive their provisional results by e-mail.

    Samples were dispatched in April 2022 and the deadline for reporting test results to the Secretariat was 1 July 2022. By this date, 95% of the 199 participants submitted their test results.

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.

    After publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Leader on issues encountered or specific questions arising.

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr


    Heterogeneity results 22-1 G.max
    Round 11 Seed Mixture seed list and images
    Summary report PT22-1 G.max

  • PT 21-3 Fagopyrum esculentum heterogeneity and summary report

    Test round 21-3 F.esc started with the shipment of three samples of Fagopyrum esculentum for purity, other seed determination, germination, and thousand seed weight.

    Participants will receive their provisional results by e-mail.

    Samples were dispatched in November 2021 and the deadline for reporting test results to the Secretariat was 1 March 2022. By this date, 94% of the 198 participants submitted their test results.

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results 21-3 F.esc
    OSD photos
    Summary report PT21-3 F.esc

  • PT21-2 Alnus glutinosa heterogeneity and summary report

    Test round 21-2 A.glu started with the shipment of three samples of Alnus glutinosa for germination test.

    Participants will receive their provisional results by e-mail.

    Samples were dispatched in November 2021 and the deadline for reporting test results to the Secretariat was 1 March 2022. By this date 83% of the 84 participants submitted their test results.

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results 21-2 A.glu
    Summary report PT21-2 A.glu

  • PT 21-2 Medicago sativa heterogeneity and summary report

    Test round 21-2 M.sat started with the shipment of three samples of Medicago sativa for purity, other seed determination, germination, and viability testing. The PT round also included reporting on the ISTA Orange Certificate as a non-rated training exercise. Participants will receive their provisional results by e-mail.

    Samples were dispatched in August 2021 and the deadline for reporting test results to the Secretariat was 1 November 2021. By this date, 96% of the 180 participants submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results PT21-2 M.sat
    OSD Photos

    Summary report PT21-2 M.sat

  • PT 21-1 Zea mays heterogeneity and summary report

    Test round 21-1 Z.may started with the shipment of three samples of Zea mays for germination, moisture, and vigour (radicle emergence) testing. Participants will receive their provisional results by e-mail.

    Samples were dispatched in March 2021 and the deadline for reporting test results to the Secretariat was 1 July 2021. By this date, 97% of the 201 participants submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel C.S.
    90024 49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results PT21-1 Z.may
    Round 10 Seed Mixture Seed List and Images
    Summary report PT21-1 Z.may

  • PT 20-3 Felicia sp. heterogeneity and summary report

    Test round 20-3 F.sp started with the shipment of three samples of Felicia sp. for germination testing.

    Samples were dispatched in November 2020 and the deadline for reporting test results to the Secretariat was 1 March 2021. This deadline was extended until 15 March 2021. By this date, 90% of the 113 participants submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results 20-3 F.sp
    Summary report PT20-3 Felicia

  • PT 20-3 Festuca pratensis heterogeneity and summary report

    Test round 20-3 F.pra started with the shipment of three samples of Festuca pratensis for purity, determination of other seeds, and germination testing. Completion of an ISTA Orange Certificate has also been requested. Participants will soon receive their provisional results by e-mail.

    Samples were dispatched in November 2020 and the deadline for reporting test results to the Secretariat was 1 March 2021. This deadline was extended until 15 March 2021. By this date, 94% of the 164 participants submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel C.S.
    90024 49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results 20-3 F.pra
    OSD photos PT20-3 F.pra
    Summary report PT20-3 F.pra


    Round 9 Seed Mixture Seed List and Images

  • PT 20-2 Allium porrum heterogeneity and summary report

    Test round 20-2 A.por started with the shipment of three samples of Allium porrum seed for purity, other seed determination, germination, viability, and thousand seed weight testing.  

    Samples were dispatched in August 2020 and the deadline for reporting test results to the Secretariat was 1 December 2020. This deadline was extended until 15 December 2020. By this date, 93% of the 180 participants had submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results 20-2 A.por
    OSD photos PT20-2 A.por
    Summary report PT20-2 A.por

  • PT20-1 Phaseolus vulgaris heterogeneity and summary report

    Test round 20-1 P.vul started with the shipment of three samples of Phaseolus Vulgaris seed for germination, moisture and vigour testing.  

    Samples were dispatched in August 2020 and the deadline for reporting test results to the Secretariat was 15 November 2020. This deadline was extended until 1 December 2020. By this date, 92% of the 182 participants had submitted their test results.   

    Heterogeneity test results are published at the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results PT20-1 P.vul
    Summary report PT20-1 P.vul

  • PT 19-3 Hordeum vulgare heterogeneity and summary report

    Test round 19-3 H.vul started with the shipment of three samples of Hordeum vulgare seed for purity, other seed determination, germination and viability testing.  

    Samples were dispatched in February 2020 and the deadline for reporting test results to the Secretariat was 27 July 2020. By this date 92% of 200 participants submitted their test results.   

    Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges-Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Seed mixture training exercise
    An extra sample with a seed mixture was included in this PT round. The participation in the seed mixture identification test is voluntary. It is an exercise organised by the ISTA Purity Committee with the aim of improving the seed identification skills of the analysts and it is not subjected to rating.

    Heterogeneity results PT19-3 H.vul
    OSD photos PT19-3 H.vul
    Summary report PT19-3 H.vul

    Round 8 Seed Mixture Seed List and Images

  • PT19-2 Brassica napus heterogeneity and summary report

    Test round 19-2 B.nap started with the shipment of three samples of Brassica napus seed for purity, other seed determination, germination and viability testing.  

    Samples were dispatched in July 2019 and the deadline for reporting test results to the Secretariat was November 1, 2019. The deadline has been extended until November 8, 2019. By this date, 93% of the 200 participants had submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PTC Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges Morel C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    PT19-2 Heterogeneity results B.nap
    OSD photos PT19-2 B.nap
    Summary report PT19-2 B.nap

  • PT19-1 Galega orientalis heterogeneity and summary report

    Test round 19-1 G.ori started with the shipment of three samples of Galega orientalis seed for purity, other seed determination, germination, and moisture testing.   Samples were dispatched in April 2019 and the deadline for reporting test results to the Secretariat was July 15, 2019. By this date, 95% of the 155 participants had submitted their test results.   

    Heterogeneity test results are provided on the ISTA website to allow the participants to review them. Heterogeneity results are the mean results of 10 samples that had been tested by the Test Leader prior to sample dispatch. The results are not necessarily the means that will be calculated but shall give a first indication of the seed quality parameters.  

    With publishing this heterogeneity test report, no further results are accepted for submission. Laboratories are kindly requested not to provide results of re-tests unless they wish to receive advice from the Secretariat or the Proficiency Test Leader on issues encountered or specific questions arising.    

    PT Chair contact data:

    Mr. Didier Demilly
    GEVES-SNES Station Nationale d'Essais de Semences
    25 Rue Georges Morel
    C.S. 90024
    49071 Beaucouzé Cedex
    FR-France
    Email: didier.demilly@geves.fr

    Heterogeneity results PT19-1 G.ori
    OSD photos PT19-1 G.ori
    PT19-1 G.ori summary report

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