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Seed Health Methods 2021

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ISTA Seed Health Methods 2021
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7 -001a: Detection of Alternaria dauci in Daucus carota (carrot) seed by blotter method

7 -001b: Detection of Alternaria dauci in Daucus carota (carrot) seed by malt agar method

7 -002a: Detection of Alternaria radicina in Daucus carota (carrot) seed by blotter method

7 -002b: Detection of Alternaria radicina in Daucus carota (carrot) seed by malt agar method

7 -003: Detection of Botrytis cinerea in Helianthus annuus (sunflower) seed

7 -004: Detection of Leptosphaeria maculans and Plenodomus biglobosus in Brassica spp. seed

7 -005: Detection of Ascochyta pisi on Pisum sativum (pea) seed

7 -006: Detection of Colletotrichum lindemuthianum in Phaseolus vulgaris (bean) seed

7 -007: Detection of Alternaria linicola, Botrytis cinerea and Colletotrichum lini in Linum usitatissimum (flax, linseed) seed

7 -008: Detection of Caloscypha fulgens in Picea engelmannii and P. glauca (spruce) seed

7 -009: Detection of Fusarium circinatum in Pinus spp. (pine) and Pseudotsuga menziesii (Douglas fir) seed

7 -010: Detection of Bipolaris oryzae in Oryza sativa (rice) seed

7 -011: Detection of Pyricularia oryzae in Oryza sativa (rice) seed

7 -012: Detection of Trichoconiella padwickii in Oryza sativa (rice) seed

7 -013a: Detection of Ustilago nuda in Hordeum vulgare subsp. vulgare (barley) seed by embryo extraction

7 -013b: Detection of Ustilago nuda in Hordeum vulgare subsp. vulgare (barley) seed by dehulling and embryo extraction

7 -014: Detection of Parastagonospora nodorum in Triticum aestivum subsp. aestivum (wheat) seed

7 -015: Detection of Epichloë coenophiala in Festuca spp. (fescue) and of Neotyphodium lolii in Lolium spp. (ryegrass) seed

7 -016: Detection of Phomopsis complex in Glycine max (soybean, soya bean) seed

7 -019a: Detection of Xanthomonas campestris pv. campestris and Xanthomonas campestris pv. raphani in Brassica spp. seed

7 -019b: Detection of Xanthomonas campestris pv. campestris in disinfested/disinfected Brassica spp. seed

7 -020: Detection of Xanthomonas hortorum pv. carotae in Daucus carota (carrot) seed

7 -021: Detection of Xanthomonas axonopodis pv. phaseoli and Xanthomonas axonopodis pv. phaseoli var. fuscans in Phaseolus vulgaris (bean) seed

7 -022: Detection of Microdochium nivale and Microdochium majus in Triticum spp. (wheat) seed

7 -023: Detection of Pseudomonas savastanoi pv. phaseolicola in Phaseolus vulgaris (bean) seed

7 -024: Detection of pea early browning virus and pea seed-borne mosaic virus in Pisum sativum (pea) seed

7 -025: Detection of Aphelenchoides besseyi in Oryza sativa (rice) seed

7 -026: Detection of squash mosaic virus, cucumber green mottle mosaic virus and melon necrotic spot virus in cucurbit seed

7 -027: Detection of Pyrenophora teres and Pyrenophora graminea in Hordeum vulgare subsp. vulgare (barley) seed

7 -028: Detection of infectious tobacco mosaic virus and tomato mosaic virus in Solanum lycopersicum (tomato) seed by the local lesion assay (indexing) on Nicotiana tabacum plants

7 -029: Detection of Pseudomonas syringae pv. pisi in Pisum sativum (pea) seed

7 -030: Detection of Acidovorax valerianellae in Valerianella locusta (corn salad) seed

7 -031: Filtration method for detection of Ditylenchus dipsaci in Medicago sativa (alfalfa); D. dipsaci and D. gigas in Vicia faba (faba bean) seed

7 -032: Detection of Verticillium dahliae in Spinacia oleracea (spinach) seed

ISTA Rules 2021 Chapter 7: Seed Health Testing

ISHI Best Practices
The ISTA Seed Health Committee recommends using the ISHI Best Practices for Seed Health Testing, but the practices are not compulsory for ISTA accredited laboratories. On the ISHI-Veg webpage look for “Method description and execution”.


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