ISTA Seed Health Committee and University of Pretoria are looking forward to hosting you at the SHC workshop on “Seed Health Methods to detect fungi, bacteria and viruses” at the University of Pretoria
Terry Aveling, Vice-Chair SHC, University of Pretoria, South Africa
Rosandya Govender, QC Pathologist, Starke Ayres, South Africa
Valerie Grimault, Chair SHC, GEVES-SNES France
Reyes Blanco - Professor at University of Almeria, Spain
Terry Aveling - Professor at University of Pretoria, South Africa
Valerie Grimault - Chair of Seed Health Committee, Head Laboratory of Phytopathology, GEVES-SNES, France
Rosandya Govender - QC Pathologist, Starke Ayres, South Africa
Aim of the workshop:
This workshop will provide an overview of Seed health testing methods and detection of seed-borne bacterial, viral and fungal pests which are very relevant for seed trade in field and vegetable crops seed industry in Southern Africa & other countries.
It will be focused on Fusarium on maize, Xanthomonas hortorum pv. carotae on carrot, Clavibacter michiganensis subsp michiganensis on tomato and Tobamoviruses on tomato.
Methods covered will be plating for fungi, dilution plating and PCR for bacteria, ELISA and indexing for viruses.
Language: English (slides and presentations)
- Organization of Seed Health Testing in South Africa (Terry Aveling)
- ISTA organization, accreditation system and SHC (Valerie Grimault)
- Pests and seed health testing methods (Valerie Grimault)
- Detection methods for
- Fusarium on maize seeds by plating on media and morphological identification (Reyes Blanco)
- Xanthomonas hortorum pv. carotae on carrot seeds by dilution plating and PCR on colonies (Valerie Grimault)
- Clavibacter michiganensis subsp michiganensis on tomato seeds by dilution plating and confirmation by PCR on colonies and pathogenicity test (Rosandya Govender)
- Tobamoviruses on tomato seeds by ELISA and indexing (Valerie Grimault)
- Best practices and limitation of the methods (interactive session: all)
- How to implement/validate your ability to perform these methods in your laboratory (Valerie Grimault)
Participation Fee: The number of participants is for a minimum of 20 and restricted to a maximum of 25 participants.
- Practical’s on
- Fusarium on maize seeds by plating on media and morphological identification
- Xanthomonas hortorum pv. carotae on carrot seeds by dilution plating and PCR on colonies
- Clavibacter michiganensis subsp michiganensis on tomato seeds by dilution plating and confirmation by PCR on colonies and pathogenicity test
- Tobamoviruses on tomato seeds by ELISA and indexing.
ISTA Members: 450 EUR
ISTA Non-Members: 675 EUR Note: The Participation fee will include all literature and supporting material for the workshop, lunches and coffee breaks, one evening workshop official dinner and transfer between the workshop venue and hotels (except airport transfer).
It does not include accommodation or meals other than those specified.Registration deadline: 15 March 2020Payment deadline: 10 days from the date invoice is issued
For cancellations made after 15 March 2020 registration fees are non-refundable.
Payment by credit card (MasterCard and Visa) is possible upon request to the ISTA Secretariat.
If you would like to attend the workshop please fill in the registration form. An invoice will be sent to you, which has to be paid before the participation confirmation will be generated. Please indicate any dietary requirements during registration. Travel & Visa information:If you require invitation letter, or for any additional information, please contact:
Direct phone: +41 44 838 68 33
Invitation Letter will be issued by ISTA Secretariat only after payment of Registration Fee.
The workshop will be held at University of Pretoria in the Plant Science Complex.
Pretoria is situated in the transitional area between the Highveld and the Bushveld, approximately 50 km north of Johannesburg in the north-east of South Africa. It lies in a warm, well sheltered, fertile valley, surrounded by the hills of the Magaliesberg range, 1,370 m (4,495 ft) above sea level with temperatures ranging from 12°C (53°F) to 20°C (68°F) (minimum and maximum, respectively in September).
Pretoria, in the Gauteng Province, is the administrative capital of South Africa. Known as "Jacaranda City" for its thousands of jacaranda trees, the city is also known for its universities and government buildings.
The semicircular Union Buildings encompass the president's offices and hosted Nelson Mandela's inauguration. Nearby is the massive Voortrekker Monument, honouring 19th-century Afrikaans settlers from the Cape Colony.
Pretoria is one of South Africa's leading academic cities, and it is home to the largest residential university in the country, the University of Pretoria with more than 62 000 students enrolled.
The university was established in 1908 as the Pretoria campus of the Johannesburg-based Transvaal University College (TUC) hence the popular name of TUKS or TUKKIES.
The university’s main campus and central administration offices are situated in the suburb of Hatfield, Pretoria and houses six of the nine faculties.
The campus is served by the Hatfield Gautrain station connecting OR Tambo airport, Pretoria and Johannesburg.
The Gautrain is an 80-kilometre mass rapid transit railway system in the Gauteng Province connecting OR Tambo with Pretoria.Suggested Accommodation
:A list of hotels is provided in annex.
Participants will be asked to book directly with the hotels. Please indicate 'ISTA Workshop' when booking and inform the local organizer; hotels will be within walking distance from the venue.