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Introduction
Participants of test round 08-2 shall receive three samples of Zea mays for germination testing and, if applicable, three samples of Portulaca grandiflora for purity, determination of other seeds and germination testing, as part of the ISTA Proficiency Test Programme for 2008-2010. In case there is any doubt as to the integrity of the samples (e.g. broken seal, inadequate quantity of seeds) the laboratory is requested to ask the Secretariat for replacement samples.
Accredited laboratories are required to participate in all tests they hold a valid accreditation for. For non-accredited tests participation is voluntary.
Purity Analysis
For each sample, a purity analysis of the whole Portulaca grandiflora sample in the envelope is required. Please carry out the analysis according to the current ISTA Rules, and report, on the attached form, the working sample weight, the weight and percentage of each component and the total weight of components. Report the initial working sample weight, the weight of components and the total weight of components in accordance with the ISTA Rules.
Other Seed Determination
Please report the number and botanical names of other seeds found in the Portulaca grandiflora samples following the ISTA List of Stabilized Plant Names, and the kind of inert matter.
Germination Analysis
For each sample, a germination analysis comprising 4 replicates of 100 pure seeds is required. Please carry out the analysis according to your laboratory's preferred ISTA alternative method, as prescribed in the current ISTA Rules. The results, including the average values for each lot are to be reported in the spaces provided on the attached form. Make sure that you indicate the germination method (substrate, temperature, counting days, treatment for promoting germination etc.) in the spaces provided on the report form (ISTA Rules Table 5A).
Reporting Results
All results shall be reported following the instructions. Please enter your laboratory member code and the random sample number on each report form.
Remaining seed shall be carefully stored for re-testing or follow-up corrective actions if required. There should be sufficient seed for one re-test.
Laboratories with an email address will receive an electronic copy of the report forms. In addition, electronic copies of the report sheets may make use of the electronic copy provided below.
Deadline for reporting results is August 31, 2008.
Treatment
The Zea mays samples have been treated with the following treatments:
Maxim XL 2.7FS, Apron XL 3LS, Dynasty 100FS and Cruiser @ 0.25
The Portulaca grandiflora samples have not been treated.
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ISTA Seed Mixture Experiment
ISTA is initiating an experiment to collect information that may be used to determine the reliability of seed mixture test results and establish tolerances.
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ISTA Annual Meeting 2009 in Switzerland
Register Online Now!
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29th ISTA Congress 2010
1st Call for Papers for the Seed Symposium of the ISTA Congress 2010 in Cologne, Germany
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Universal List of Species
ISTA has published a list of 130 plant species that accredited laboratories may wish to include in their seed collections
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SEED TESTING INTERNATIONAL
(ISTA News Bulletin) No. 136 October 2008 for download
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Accreditation Documents for the Testing of Specified Traits
Related Documents for download
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ISTA / ISF Experiment on Herbage Seed Lot Size
The ISTA / ISF Experiment on Herbage Seed Lot Size
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ISTA signs Memorandum of Understanding with FAO
Read the full Press Release
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