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ISTA SHC Workshop: Seed Health Methods using PCR, ELISA, dilution plating and indexing methods

       
 

Date:

20.11.2018 - 23.11.2018


Location:

Bangalore, India


Kindly note that the workshop is postponed to 20-23 November 2018.


ISTA Seed Health Committee and Indo American Hybrid Seeds(India)Pvt., Bangalore are looking forward to hosting you at the "ISTA SHC Workshop: Seed Health Methods using PCR, ELISA, dilution plating and indexing methods" in the Seed Laboratory IN07.


Organizers:

Dr. G. V. Jagadish
- Head and Member, ISTA Vigour Committee
Dr. Devaraja Achar - Head, Biotechnology and Member, ISTA Advanced Technology Committee
Dr. B. Mahesh - Pathologist- Seed Health laboratory

Seed Laboratory IN07
Indo-American Hybrid Seeds (India)Pvt.Ltd.
7th km, Banashankari-Kengeri Link Road,Kengeri Hobli, Channasandra
Rajarajeshwari Nagar Post, Bengaluru-560 098, India

E-Mails: jagadish@indamseeds.com / mahesh@indamseeds.com / devaraja.achar@indamseeds.com
Website: www.indamseeds.com


Lecturers:

  • Valerie Grimault - Chair of ISTA Seed Health Committee, specialized on phytopathology, France
  • Thomas Baldwin, specialized on PCR, France
  • Corinne Sarniguet, specialized on nematodes, France
  • Fadhilah Siti, specialist of Aphelenchoides, Indonesia

Aim of the workshop:

This workshop will provide an overview of Seed Health Testing Methods and detection of seed-borne bacterial,viral and nematode pests which are very relevant for seed trade in the field and vegetable crops seed industry in India & other countries.
It will be focused on Xanthomonas campestris pv. campestris on Brassica spp., Clavibacter michiganensis and Tobamoviruses on tomato, Cucumber Green Mottle Mosaic Virus CGMMV cucumber and Aphelenchoides on rice.
Methods covered will be dilution plating, ELISA, SE-PCR and PCR on isolates and morphological identification.


Language: English (slides and presentations)

Target group: The workshop is aimed for Seed Analysts and Quality managers of seed companies and seed trade officials and plant quarantine department of the private and Government officials, Seed Scientists in the Government Institutions and Agricultural Universities.


Preliminary program:

Lectures
  • Organization of Seed Health Testing in Afro-Asia by Dr.H.S.Prakash - Chair, Department of Biotechnology, Univ. Of Mysore/India
  • ISTA organization, accreditation system and SHC.
  • Pests and seed health testing methods
  • Detection methods for
    • Xanthomonas campestris pv. campestris on Brassica spp. By dilution plating and confirmation by PCR on colonies and by seed extract PCR (Taqman)
    • Cucumber Green Mottle Mosaic Virus CGMMV cucumber by ELISA
    • TMV by indexing on tobacco plants (biotest)
    • Clavibacter michiganensis on tomato by dilution plating and confirmation by PCR on colonies and pathogenicity test
    • Aphelenchoides on rice by morphological identification
  • Best practices and limitation of the methods
  • How to implement/validate your ability to perform these methods in your laboratory
Practical’s on
  • Xanthomonas campestris pv. campestris on Brassica spp. By dilution plating and confirmation by PCR on colonies and by seed extract PCR (Taqman)
  • Cucumber Green Mottle Mosaic Virus CGMMV cucumber by ELISA
  • TMV by indexing on tobacco plants (biotest)
  • Aphelenchoides on rice by morphological identification.
Visit to Botanical gardens Lal Bagh from 4pm to 7pm on 3rd day of the workshop



Location:

Seed Laboratory IN07
Indo-American Hybrid Seeds (India) Pvt. Ltd.
Bengaluru (Bangalore), India


Accommodation:   
 
Participants are asked to book accommodation directly with the hotels. Please indicate 'ISTA Workshop' when booking and inform the local organizer.  
   
Suggested Accommodations:  

Hotel La Marvelle - 14th Cross, South end circle,2nd Block, Jayanagar, Bangalore- 560011, India
Tel: +91-80-4333 5333. 
Email: info@lamarvella.com   
Price for Single room including breakfast: approx. 6 013 INR (Euro 78 ) + taxes.     

Hotel President - Elephant Rock Road, Jayanagar3 rd Block, Bangalore - 560 011
Tel: +91 80 4180 8777
Email: gmsales@paihotels.com 
Price for Single room including breakfast: approx. 4 876 INR (Euro 64) + taxes.  
 
Hotel  Pai Viceroy - 1504, 16th cross, 9th Main, Jayanagar3 rd Block, Bangalore - 560 011
Tel: +91 088920 77889
Email: gmsales@paihotels.com
Price for Single room including breakfast: approx. 3 600 INR (Euro 47)    
             Double room including breakfast: approx. 4 100 INR (Euro 54)     


Travel & Visa information: Letter of invitation for obtaining visa and to travel is issued for all registered participants only after payment of the participation fee.


Participation Fee:  

The number of participants is a minimum of 20, without which the workshop will be cancelled and limited to a maximum of 25 participants.

ISTA Members: 500 EUR

ISTA Non-Members: 750 EUR

Note: The Participation fee will include all literature and supporting material for the workshop, lunches and coffee breaks, workshop dinner and transfer between the workshop venue and hotels (except airport transfer). It does not include accommodation or meals other than those specified.

Registration deadline: 20 August 2018

If you would like to attend the workshop please fill in the registration form. An invoice will be sent to you, which has to be paid before the participation confirmation will be generated. Please indicate any dietary requirements during registration. 
All payments are required by 28 September 2018

Please note: 

Payment by credit card (MasterCard and Visa) is possible upon request to the ISTA Secretariat.     

For cancellations made before 20 August 2018 registration fees are refundable less 50 Euro Administration Fee
For cancellations made after 20 August 2018 registration fees are non-refundable



About Bangalore & Indo American Hybrid Seeds
  • Bangalore (or  Bengaluru) is the capital city of the Indian state of Karnataka. Bangalore is India's third most populous city and fifth-most populous urban agglomeration. Located at a height of over 3,000 feet (914.4 m) above sea level, Bangalore is known for its pleasant climate throughout the year. The city is amongst the top ten preferred entrepreneurial locations in the world.
  • Indo American Hybrid Seeds, Bengaluru, India has a seed testing laboratory IN07, and was the first private laboratory in India in 2002 to be accredited by ISTA.
  • The core business of Indo American Hybrid Seeds is production and marketing of hybrid seeds and the company established a seed laboratory for testing seed for purity, germination, and vigour. The Company also has a seed health laboratory and a molecular diagnostics laboratory. It has been in business serving Indian and international clients since 1965.
  • More information about Indo American Hybrid Seeds, Bengaluru can be found at www.indamseeds.com


 


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