ISTA Workshop on Variety Testing - an introduction, using protein electrophoresis and Polymerase Chain Reaction (PCR) for GMO Detection

Kindly note that the workshop was originally planned for November 2009 and has been now postponed to April 06-10, 2010.

Workshop Content

The workshop shall provide general information regarding the applications and hand-on experience of electrophoresis and PCR-based methods in the fields of variety verification and GMO detection.
These will cover:

Statistics:  Use SeedCalc to develop GM and conventional seed lot testing plans and interpret associated results.

Variety verification:

• Object, field of application and general principles of the verification of species and cultivars
• Seed storage proteins analysis
• Testing of seed storage proteins with IEF, PAGE; SDS; Isozymes: Protein extraction, gel preparation up to the evaluation of the gels  

Practical Work:

Isoelectric Focusing in Ultrathin layer (IEF) of seed storage proteins: Zea, Triticum, Oryza, Capsicum; Isozymes : ZeaExtraction of different protein classes,

• Production, loading and running of gels
• Evaluation of results
• ISTA Method for maize and sunflower and adaptation of methods for special questions

 PCR testing:

• A primer on sampling and testing design for Low Level Presence of biotech seed
• DNA Isolation and quantification
• Introduction to the polymerase chain reaction and its applications
• Introduction to Real-time PCR
• Practical considerations in setting up a PCR lab.

Hands-on Lab will include:

• DNA isolation, DNA quantification
• Qualitative PCR of 35s promoter and endogenous gene control in maize.
• Quantitative, event specific PCR of transgenic corn.
  
  

Local Organiser

Dr. G.V.Jagadish
Indo-American Hybrid Seeds (India) Pvt. Ltd
Seed Laboratory
7th km, Banashankari-Kengeri Link Road
Bangalore
India
jagadish@indamseeds.com
Phone: +91 80 2864499
Fax: +91 80 28602912


Lecturers

Variety identification: Norbet Leist, Rainer Knoblauch
PCR Testing: Benjamin Kaufman
Statistics, sampling and data analysis: Kirk Remund


Registration

Number of participants: 20
Registration fees: Member: 600 €    Non-member: 700 €

Deadline for registration: March 2, 2010

Bank details:

Indo-American Hybrid Seeds (India)
Pvt LtdOur A/C No. 0408261010631
At
Canara Bank
Jayanagar Shopping Complex Branch
4^th Block ,Jayanagar
Bangalore-560011 (India )
Tel:00-91-080-26630880

THROUGH:
Canara Bank
Foreign Department
44/45, Residency Cross Road
Bangalore-560025
SWIFT CODE: CNRBINBBLFD
Tel:00-91-080-25582520

Routing Details of TELEX TRANSFER:
By crediting the amount to U.S.D. Account No. 001-1-395969 with chips UID-107777 with the CHASE MANHATTAN BANK , NEW YORK maintained by Canara Bank, Bangalore for further  credit to Canara Bank Shopping Complex Branch, Bangalore-560011 to the current Account No.0408261010631 of Indo-American Hybrid Seeds (India) Pvt Ltd., Bangalore by Swift –CNRBINBBLFD through MT-100 Routing No. 021000021. Please fax us the copy of the payment details for our follow  up.


Accommodation

The President
# 79/8, Diagonal Road
(Elephant Rock Road)
3rd block, Jayanagar
Bangalore -11
Tel:080 41808777
Fax:080 41808700
www.presidenthotel.in
enquiry@presidenthotel.in

Comfort Single (47 €)
Comfort Double (54 €)
Luxury Single (67 €)
Luxury Double (74 €)
Studio Single/Double Room (80 €)
Extra Bed (10 €)

Hoysala
No. 212
Subedar Chatram Rd
Seshadripuram
Tel:23464300; 23464301; 23464307;M. 9845291253
Fax: 080 23316097
www.hotelhoysala.com
hotelhoysala@mantraonline.com

Single Room (11 €)
Double room non-air condition (18 €)
Double Room air condition (24 €)

Hotel Chancery
Lavelle Road
Bangalore
www.chanceryhotel.net

Single Room (95 €)
Double Room (112 €)