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Numbered sections appearing in bold are obligatory for OICs.
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1. Choose the appropriate Certificate
International Seed Lot Certificate: Orange Certificate
International Seed Sample Certificate: Blue Certificate
2. Laboratory stamp
Stamp of issuing laboratory
3. Name of applicant
Name and address of the applicant has to be stated on Seed Sample Certificates (BIC). It may be omitted on Orange (OIC) Certificates upon request by the applicant (17.4.1.1).
4. Species, cultivar, category, weight of lot etc.
Information supplied by the applicant; the laboratory has no responsibility for the correctness of these but must quote them as stated by the applicant. If the lot size of coated seeds is stated in terms of units, the weight must be given as well.
5. Testing and issuing laboratory
Name, address and ISTA member code of the issuing laboratory as it is registered with ISTA and given on the ISTA website.
6. Sampling by (not applicable for BIC)
Name and ISTA member code of the laboratory responsible for the sampling, if different from the issuing laboratory (17.4.1.2.b).
7. Marks of lot
Seed lot designation for each particular container of the lot, e.g. the label numbers used for the seed lot (2.2.10, 17.4.1.2).
8. Seal of lot (not applicable for BIC)
It is at the laboratory's discretion what to report under "seal of the lot".
9. Status of Certificate
'ORIGINAL', 'DUPLICATE' as applicable (17.2.3 and 17.3 g).
10. Number of containers (not obligatory for BIC)
Number of containers the seed lot is kept in at the time of sampling and sealing.
11. Date of sampling (not applicable for BIC)
Date of sampling in the format yyyy-mm-dd (17.4.1.1.b). This format has to be followed in all places where a date is used on the OIC or BIC.
12. Date sample received
Date when the sample was registered at the testing laboratory. Format yyyy-mm-dd (17.4.1.1.b).
13. Date test concluded
Date of the termination of the last test reported on the Certificate.
14. Test number
Testing laboratory's unique test or sample number (17.4.1.2.h).
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15. Species
The species name has to be stated in accordance with the Table 2A of the ISTA Rules with the exception that the genus name may suffice if the species cannot be determined from the seed characteristics (17.4.1.1.c). If applicable, it shall be followed by a description for the kind of coated seeds, 17.5.3.4). In the case of coated seeds, the words 'seed pellets', 'encrusted seed', 'seed granules', 'seed tapes' or 'seed mats' have to be stated as applicable.
16., 17., 18. Purity
Percentage (weight) of pure seed fraction, inert matter and other seeds to one decimal place, '0.0' if the result from the analysis is nil, Trace ('TR') if it is less than 0.05 % (3.7, 17.5.3.1).The percentage of all components must total 100.0. If no determination has been made the entry shall be 'N'.
Individual impurities with an undue effect on the purity results have to be reported in accordance with 3.6.5.
19. Number of days
Duration of the germination test, excluding any pre-treatment period (5.9). If no determination has been made, the entry shall be 'N'.
20., 21., 22., 23., 24. Categories
Results are reported to the nearest whole number as average of four 100 seed replicates, '0' if there are none in the particular category. The entries must add up to 100. If no determination has been made or the space provided is not applicable, the entry must be 'N'.
An 'N' must be reported in all the spaces provided for reporting the percentages of the components of the germination tests when results of weighed replicates are reported.
25. Moisture content
The percentage is reported to one decimal place (9.1.7, 9.2.2.7). If no determination has been made the entry must be 'N'.
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26. Kind of inert matter
The kind of inert matter from the purity analysis must be identified and reported. When on request of the applicant, the content of a particular kind of inert matter has been determined, the percentage of such material must be shown on the ISTA International Seed Analysis Certificate (3.7, 17.5.3.1).
27. Other seeds
The other seeds retrieved during the purity test have to be stated individually by their scientific names according to Table 2A or, where applicable, in accordance with the current ISTA List of Stabilized Plant Names available at www.seedtest.org. If required by the applicant, 'Other seeds' may be divided into 'Other crop seeds' and 'Weed seeds'. In that case, the words 'Other crop seeds' must be entered, followed by the percentage by weight for other crop seeds and the name(s) of the species found. This procedure must also be used for 'Weed seeds'. (17.5.3.1). When requested, the percentage of a particular species of other seed shall be determined and reported on the Certificate (3.7).
28. Other determinations
If a provisional Certificate is issued, the Certificate shall include a statement that this is a 'Provisional' Certificate and that the final Certificate will be issued upon completion of a test or tests (17.2.4).
References to particular legal requirements and whether they are met by the lot are at the laboratory's own risk (17.5.3.10).
Results of tests not covered by the ISTA Rules. The method must be stated, followed by: "(this method is not covered by the International Rules for Seed Testing)" (17.5.3.9).
In case a previous Certificate has to be declared void, all relevant details have to be given including the passage: "This lot was originally sampled …" (17.6).
Purity
- In case the quantity used for the purity analysis was less than stipulated by Table 2A, the statement under 2.5.4.4 "The sample submitted weighed only ….. g [or in the case of pelleted seeds 'contained only .... pellets (seeds)'] and is not in accordance with the International Rules for Seed Testing."
- When required, the percentage of multiple seed unit (MSU) or seed with appendages attached shall be determined and reported on the Certificate (3.7, 17.5.3.1).
- Additional information about the purity test when it is difficult to distinguish between species and the procedure described under 3.5.2.4.bmust be stated (3.5.2.4).
- As part of the purity analysis of coated seeds the percentage and names of other species found in 100 seeds after removal of the coating material are to be stated. The percentage of pelleting material shall be reported separately only on request (11.3.5.A).
- If the weight of the purity working sample deviates from that described in Table 2A e.g. (3.7).
Other seed by number
- The results from Other Seed by Number are reported with the scientific name, the number of seeds for this species and the actual sample weight examined. In addition, the result may be expressed differently (e.g. .#/kg). The intensity of the test has to be stated as 'COMPLETE', 'LIMITED', 'REDUCED' or 'REDUCED-LIMITED' test (4.7 and 17.5.3.2).
Germination
- If germination test results are reported, the germination conditions (temperature, substrate, treatments) shall be stated using the abbreviations used in Table 5A. The germination percentage within the prescribed period if the period had been extended (i.e. "After the prescribed period of ... days there were ... % normal seedlings") and the second result where duplicate (double) tests are indicated in Table 5A. Duration and kind of treatment to promote germination, name of chemical and percentage of active ingredients in case of disinfection of seed (5.6.3, 5.6.3.4, 5.9, 17.5.3.3).
- Upon request: results of any additional test, viability of ungerminated seeds and method used to determine it, categories of ungerminated seeds and the methods to determine them, details about multigerm units (5.9., 17.5.3.3).
Tetrazolium test
- Tetrazolium test: "… % of seeds were viable." Percentage of hard seeds for Fabaceae. In the cases where the testing procedure deviates from the prescription in Table 6A, the respective data must also be reported (6.7).
- In the case of species of the Fabaceae (Leguminosae) the percentage of hard seeds found in the test or the percentage of hard seeds included in the percentage of viable seeds shall be reported (6.7).
- Further details may be given at the discretion of the testing station (6.7, 17.5.3.6 ).
Seed health
- Percentage of infected seeds and scientific name of organisms for seed health tests, including method and pre-treatment. The amount of seeds or the fraction of the sample examined has to be stated. Additional directions regarding reporting are given in the individual methods (7.6, 17.5.3.7).
Moisture
- When using the constant temperature oven method the duration and the temperature must be reported.
- Additional information on the condition of the seeds or regarding pelleting must be reported as applicable (9.1.7 and 9.2.2.7).
Weight determination
Results shall be reported as average weight of 1,000 seeds (10.6).
Excised embryo
Results shall be reported in the following form:
Excised embryo test: "... % of seeds had viable embryos."
Further details may be given at the discretion of the testing station, e.g. percentage empty, insect or physically damaged (12.7).
Weighed replicates
The laboratory shall report, the average weight of the four replicates, the average number of normal seedlings in four replicates, the number of normal seedlings per kg and other information as stated under 5.9 (13.7)
X-Ray
Results shall be reported as (14.7):
"X-ray test results:
... % filled
... % empty
... % insect-damaged
... % physically damaged".
Vigour
Conductivity: "Conductivity: ... μS cm-1g-1"
Accelerated ageing: "Accelerated ageing: ... % germination"
Specific variables (time, temperature, seed moisture) must be reported when appropriate.
29. Place and country
State place and country of issue, e.g. Edinburgh, UK
30. Date
Date of issuance of the Certificate. Format yyyy-mm-dd (see 11).
31. Signature
Signature of the head of the issuing laboratory or their assignee(17.6).
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32. Additional observations (on the verso)
Any continuation of results from 28 or other observations.
When the seed lot is located in a different country to the sampling laboratory, the country where the seed lot has been sampled must be reported (17.4.1.2).
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