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Working Groups
Working Group 1, Accelerated Ageing; Wheat
Leader: J. Hampton
Members: D. Bell, E. Casarini, S. H. Kim, M. Kruse, F. Kryzanowski, A. Prevaux
Working Group 2, Accelerated Ageing; Soyabean
Leader: D. TeKrony
Members: T. Gutormson, J Hampton, C. Leonhardt, M.-H. Wagner
Working Group 3, Accelerated Ageing; Maize
Leader: D. TeKrony
Members: D. Bell, E. Casarini, J. Hampton, C. Leomhardt, S. H. Kim, F. Kryzanowski
Working Group 4, Conductivity; Soyabean
Leader: A. A. Powell
Members: A. Biddle, D. Bell, E. Casarini, J. Hampton, F. Kryzanowski, J. Mackie, D. TeKrony, M.-H. Wagner, Y. R. Wang
Working Group 5, Tetrazolium; Maize
Leader: J. Franca Neto;
Members: E. Asiedu, E. Casarini, M. Dadlani, P. Garreau, M. Kruse
Working Group 6, Controlled Deterioration; Oilseeed rape
Leader: A.A. Powell;
Members: E. Casarini, H. Elberling, M. Kruse, D. Spencer, M.-H. Wagner
Activity Report
Proficiency test for completion of the Conductivity test for Pisum sativum (TOR 3)
A report on the test results has been sent to the participants. There was good repeatability of the test results within and between test runs in each laboratory and also between laboratories. All results reported fell within the previously determined tolerance levels for two of the three lots tested. The third seed lot was of much poorer quality than initially believed and the conductivity very high being greater than the levels that would normally be considered acceptable for commercial seed. Hence the mean conductivity did not occur within the tolerance tables established. Even so, the repeatability within and between laboratories was good.
Joint ISTA/AOSA Vigour Test Handbook (TOR 4)
A timetable for the production of a joint handbook is being prepared for in collaboration with the joint chairpersons of the AOSA Vigor Committee. This is planned to be available by May 2004.
Extension of the application of the Conductivity and Accelerated Ageing tests (TOR 7)
The main focus of the work has been on the expansion of the crop species database.
1. Working Group 1 Accelerated Ageing, Wheat. This comparative programme has been delayed to allow confirmation of the most suitable conditions for testing wheat. This has been taking place in the laboratory of the group leader, J Hampton. Currently conditions of 41oC for 72h appear most suitable, but there is a potential problem with dormancy after harvest. The conditions for AA testing of perennial ryegrass (43oC, 72h), garden and field pea (41oC, 72h) and Brassica oleracea var acephala (41oC, 48h) have also been established.
2. Working group 3, Accelerated Ageing, Maize. The comparative programme aimed to extend the AA test both to a new species and to laboratories outside the United States. Seeds were distributed to 13 laboratories in September 2003. These laboratories included 6 ISTA laboratories (AT, NZ, IT, US, DE, FR), 2 AOSA laboratories and 5 SCST laboratories. Problems were unfortunately encountered in their distribution from the United States and two sets of samples were sent out, the second through the ISTA Secretariat. Even when distribution was successful, there was a delay of two months in the receipt of some samples. All test results will be returned by December 2003 and will be available at the ISTA meeting in 2004.
3. Working group 4, Conductivity, Soyabean. Michael O'Neill, from Pioneer Hi-Bred International in the United States, is currently preparing seeds for distribution to the participants. Each laboratory will test up to five seed lots in each of three test runs, following the conductivity procedure validated for Pisum sativum. It is hoped that the results of this comparative test will be available at the ISTA meeting in 2004.
Standardisation of new vigour test (TOR 7)
1. Working group 6, Controlled Deterioration, Oilseed rape. A report of the test results has been sent to all participants. As in previous years the repeatability of results was good both between runs within a laboratory and between laboratories. Two alternative procedures for raising seed moisture content were compared. The first, using imbibition on filter paper is a standard method, the second involved tumbling seeds in a rotating container following the addition of a specified amount of water. The results using the latter method were less consistent between laboratories, probably because each laboratory used equipment that was available to it, and this differed. The filter paper had similar vigour levels as indicated by their CD results and field emergence at one site.
Future Aims
Proficiency tests
A second round of proficiency testing will be initiated for the Conductivity test for Pisum sativum
ISTA / AOSA Handbook
Initial steps for the revision of the handbook will follow the timetable produced.
Vigour Workshop
Presentation of a workshop in Lanzhou, China in August 2004.
Application of Accelerated Ageing and Conductivity tests.
Extension of the species database of these two tests will focus on two species, AA for maize and conductivity for soyabean.
Standardisation of new vigour test
Material and laboratories will be organised, and documentation prepared, for the submission of the Controlled Deterioration test to the ISTA Method Validation Programme as a new vigour test for Brassica species |
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ISTA Seed Mixture Experiment
ISTA is initiating an experiment to collect information that may be used to determine the reliability of seed mixture test results and establish tolerances.
More info »
ISTA invites participants to register for its 11th GMO Proficiency Test
on Canola/Oil Seed Rape (Brassica sp.)
More info »
ISTA Annual Meeting 2009 in Switzerland
Register Online Now!
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29th ISTA Congress 2010
1st Call for Papers for the Seed Symposium of the ISTA Congress 2010 in Cologne, Germany
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Universal List of Species
ISTA has published a list of 130 plant species that accredited laboratories may wish to include in their seed collections
More info »
Accreditation Documents for the Testing of Specified Traits
Related Documents for download
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ISTA / ISF Experiment on Herbage Seed Lot Size
The ISTA / ISF Experiment on Herbage Seed Lot Size
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ISTA signs Memorandum of Understanding with FAO
Read the full Press Release
More info »
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